|
KMID : 0545120030130060906
|
|
Journal of Microbiology and Biotechnology
2003 Volume.13 No. 6 p.906 ~ p.911
|
|
|
Functional Analysis of Spectinomycin Biosynthetic Genes from Streptomyces spectabilis ATCC 27741
|
|
JO, YOU-YOUNG
KIM, SUN-MEE/YANG, YOUNG-YELL/KANG, CHOONG-MIN/SOHNG, JAE-KYUNG/SUH, JOO-WON
|
|
|
Abstract
|
|
|
|
The function of genes related to spectinomycin biosynthesis (spcD. .speA, speB, spcS2) from Streptomyces .spectabilis ATCC 27741, a spectinomycin producer, was analyzed. Each gene was subcloned from a spectinomycin biosynthetic gene cluster and ovcrexpressed in E. c d i BL21 (DE.3) using PET vector. After incubating each purified protein with its possible substrates, the final products were analyzed using high-performance liquid chromatography (HPLC). From these results, spcD, speA, and .spB have been identified to be dTDP-glucose synthase, myo-inositol monophosphatase. and myo-inositol dehydrogenase, respectively. In addition, the results suggest that the .spcS2 gene product functions downstream of the s p B gene product in the biosynthetic pathway of spectinomycin. Taken together, the present study elucidates the early steps of the biosynthetic pathway for 6-deoxyhexose (6-DOH) part (actinospectose) and aminocyclitol part (actinamine) of spectinomycin.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|